麥康凱瓊脂培養基

更新時間：2016-06-11

簡要描述：

麥康凱瓊脂培養基，年中放價銷售了，低價、*、優惠價供應，微生物培養基折扣價提供，BD培養基、Gibco培養基、Amresco培養基、OXOID培養基等國內外培養基，我們傾情提供。

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麥康凱瓊脂培養基

MacConkey Agar

250g

用于腸道致病菌的選擇性分離培養。

用途：用于腸道致病菌的選擇性分離培養

麥康凱瓊脂培養基配方：（g/L）

蛋白胨
乳糖
牛膽鹽
氯化鈉
中性紅
瓊脂
PH值

20.0g
10.0g
5.0g
5.0g
0.03g
14.0g
7.1±0.2

原理：

蛋白胨提供碳氮源、維生素和生長因子；牛膽鹽可抑制革蘭氏陽性菌的生長；氯化鈉維持均衡的滲透壓；瓊脂是培養基的凝固劑；乳糖為可發酵的糖類；中性紅是pH指示劑，細菌發酵乳糖產酸時菌落呈粉紅色并在菌落周圍出現膽鹽沉淀的渾濁圈。

用法：

稱取本品54克,加熱溶解于 1000ml蒸餾水中，121℃高壓滅菌15分鐘備用。

質量控制：

在36±1℃培養18-24小時。

質控菌株	菌株編號	生長情況	其它特征
金葡萄球菌	ATCC25923	-	/
大腸埃希氏菌	ATCC25922	+++	紅色菌落、有膽鹽沉淀環
沙門氏菌	ATCC14028	+++	無色大菌落

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